Cryo-Electron Microscopy in Structural Biology - Appasani, Krishnarao; (ed.) - Prospero Internet Bookshop
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Cryo-Electron Microscopy in Structural Biology

From Structural Insights to Tomography and Drug Discovery
 
Edition number: 1
Publisher: CRC Press
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Short description:

Cryo-Electron Microscopy, in combination with Tomography, has emerged as a new technology for visualizing molecular structures revealing the native molecular details of membranes, viruses, and cells. This comprehensive, volume brings together authoritative overviews of these methods from structural and biological perspective.

Long description:

Cryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 ?. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological perspectives. It is a must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, structural biology, crystallography, and genomics.



Key Features



? Focuses on the applications of cryo-EM to structural biology


? Documents the importance of cryo-EM/ET approaches in studying the structural determinants of cellular organelle and membrane protein biochemistry


? Reviews the applications of high-resolution structures of viruses


? Emphasizes structural insights of nuclear and gene machineries


? Includes a section focused entirely on the applications of cryo-EM/ET in drug discovery and therapeutic development

Table of Contents:

Editor


List of contributors


Foreword


Preface



SECTION I Cryo-EM in Cell Biology: Structure Determination of Protein Assemblies and Cellular Organelle



Chapter 1 Introduction on Cryogenic Electron Microscopy in Structural Biology


Krishnarao Appasani



Chapter 2 Structural Dynamics of Proteasome Regulation by Cryo-EM


Youdong Mao



Chapter 3 Cryo-EM Structures of a Ubiquitin-Specific Protease in Complex with Substrate


Martin Rennie



Chapter 4 Structural Biology of Protein Kinases and Their Regulation


Samrat Mitra, Pranita Ray, Prateeka Borar, and Smarajit Polley



Chapter 5 The Life and Times of p53: Structural Advancements to Decipher the Guardian of the Genome


Maria J. Solares, Samantha Berry, and Deborah F. Kelly


SECTION II Technology Platform and Evolving Technologies


Chapter 6 Single-Particle Cryo-EM: What Happens Inside the Black Box?


Sayan Bhakta and Marin van Heel



Chapter 7 Streptavidin Affinity Grids in the Study of Macromolecular Complexes Using Cryo-Electron Microscopy


Nils Marechal and Patrick Schultz



Chapter 8 Running a Cost-Effective Modern Cryo-EM Facility for SPA Analysis


Lijia Jia



Chapter 9 Computational Methods for Bimolecular Structure Modeling for Cryo-EM


Javad Baghirov, Xiao Wang, Genki Terashi, Shu Li, and Daisuke Kihara



Chapter 10 Artificial Intelligence-Driven Data Acquisition for Single-Particle Cryo-EM and Three-Dimensional Electron Diffraction


Koji Yonekura


Chapter 11 From Icy Specimens to Digital Insights: Integrating Sample Preparation, Data Analysis, and IT Infrastructure


Eric Shell, Karine Minari, and Vitor Hugo Balasco Serr?o


SECTION III Cryo-EM in Virology and Enzymology: High-Resolution Structures of Chaperonins and Enzymes



Chapter 12 Cryo-EM Structures of Viral and Bacterial Chaperonins


Olga S. Sokolova and Tatiana B. Stanishneva-Konovalova



Chapter 13 Cryo-EM Structure of Monkeypox Virus DNA Polymerase Holoenzyme


Yi Shi and George F Gao



Chapter 14 Determining the High-Resolution Structure of the [NiFe]-hydrogenase Huc by Native Host Purification and Cryo-EM


Hari Venugopal, Ashleigh Kropp, Chris Greening, and Rhys Grinter



Chapter 15 Membrane Rupture and Lipid?Protein Interaction of ?-Hemolysin from Staphylococcus aureus Using Cryo-EM


Suman Mishra, Anupam Roy, Arnab Chatterjee, and Somnath Dutta


SECTION IV Cryo-EM in the Study of Membrane Biology: Probing the Structures of Membrane Proteins



Chapter 16 Historical Evolution and Perspective on Cryo-EM Analysis of Membrane Proteins


Youzhong Guo



Chapter 17 Single-Particle Cryogenic Electron Microscopy Unfolds Distinct Membrane Transporter


Folds: Focus on Autoinducer-2 Exporter Family


Radhika Khera



Chapter 18 Rhodopsin Dimers in the Retinal Rod Outer Segment


Sahil Gulati and Krzysztof Palczewski



Chapter 19 Cryo-EM Structures of the Fungal GPCR Dimer Ste2


Vaithish Velazhahan and Christopher G. Tate



Chapter 20 Human V-ATPases: Assembly, Function, and Regulation


Elizabeth Fosuah, Qingpeng Lin, and Tian-Min Fu



Chapter 21 Structure and Function of the Intracellular Calcium Channel Ryanodine Receptor


Marco C. Miotto



Chapter 22 Cryo-EM of Mitochondrial Membrane Proteins


Swati Yadav, Shaileshanand Jha, and Kutti R. Vinothkumar


SECTION V Cryo-EM in the Study of Molecular Biology: Structural Insights of Gene Machineries



Chapter 23 Imaging Structurally Dynamic Ribosomes with Cryogenic Electron Microscopy


Samantha M. Webster, Mira B. May, Barrett M. Powell, and Joseph H. Davis



Chapter 24 Cryo-EM in the Study of Ribosome Biogenesis


Ankita Arora and Prem S. Kaushal



Chapter 25 Cryo-EM Structures of Ribosomes from Human Pathogens: Implications for Drug Targeting


Ankit Dhur, Aneek Banerjee, Krishnamoorthi Srinivasan, and Jayati Sengupta


SECTION VI Cryo-EM in the Study of Neurobiology and Skin?Neuroreceptors to Ion Channels and Synaptic Transmission



Chapter 26 Cryo-EM Structural Features of Glutamate Receptors Involved in Synaptic Neurotransmission or Brain Pathology


Nebojša Bogdanović



Chapter 27 Cryo-EM Structures of Systemic Amyloid Fibrils


Binh An Nguyen, Maria del Carmen Fernandez-Ramirez, Shumaila Afrin, and Lorena Saelices



Chapter 28 Structural Insights into the 5-HT3A Receptor Revealed by Cryogenic Electron Microscopy (Cryo-EM)


Zhuowen Li, Nikhil Bharambe, and Sandip Basak



Chapter 29 Molecular Cryo-EM in the Study of Skin Tissue


Lars Norlén


SECTION VII Applications of Cryo-ET and Cryo-EM in the Biopharmaceutical Industry



Chapter 30 Emerging Technologies in Cryo-Electron Tomography


Kanika Khanna



Chapter 31 Cryo-Electron Tomographic Imaging of Membrane Proteins from Mitochondria and Chloroplasts


Tofayel Ahmed and Antara Saha


Chapter 32 Functional Studies of the Actin Cytoskeleton by Cryogenic Electron Tomography


Dorit Hanein and Niels Volkmann



Chapter 33 Cryo-EM in Drug Discovery


Youxin Kong, Maxime Killer, Madhumati Sevvana, and Chiara Rapisarda



Chapter 34 Applications of Single-Particle Analysis and Cryo-ET in Drug Discovery


Qing Yao



Index