| ISBN13: | 9789819745524 |
| ISBN10: | 9819745527 |
| Binding: | Hardback |
| No. of pages: | 487 pages |
| Size: | 235x155 mm |
| Language: | English |
| Illustrations: | 4 Illustrations, black & white; 58 Illustrations, color |
| 700 |
Flow Cytometry
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Flow cytometry is a state-of-the-art technology that is widely used in biological research and clinical diagnostics. It is a high-throughput platform that allows the quantification, differentiation, and functional study of cells or cell like particles in suspension. Various cell components can be targeted with fluorescently labelled antibodies or fluorescent dyes to allow measurement and analysis of the physical, chemical, and biological properties of individual cells within homogenous or heterogeneous populations.
Since its commercialization more than five decades ago, flow cytometry has advanced and become an very important analytical tool in translational research. With the advancement of instrument technology, an increasing number of fluorescent dyes, and an expanding range of monoclonal antibodies, the applications of flow cytometry in applied research continue to grow.
The use of multiparametric flow cytometry in translational research provides the ability to rapidly identify different cell populations and to simultaneously measure multiple parameters of single cells for efficiently assessing immune status, decrease/increase of specific immune cell populations, cell activation status, etc. of different cells in preclinical and clinical studies.
The primary goal of any research work is to take research results from the "bench to-bed and back". The use of flow cytometry in applied research advances the development of new diagnostic tests or drugs for cancer treatment, immune monitoring, etc. that help in patient care.
Today, every biological scientist needs to have basic knowledge of flow cytometry in order to utilise this technology properly in their own research and to understand other?s research work. The present book has been designed to give the knowledge of flow cytometry and its applications to the researchers and teachers. It will allow the readers to utilize the technology in an appropriate way in their research work. This book has describing various applications of flow cytometry like cell health monitoring, immunophenotyping, cell sorting, stem cell characterization, micro-vesicle analysis etc.
Chapter 1. Flow cytometry: historical perspectives, fundamentals, past and present instrumentations and applications.- Section 1. Instrumentation.- Chapter 2. Instrumental and experimental quality control in flow cytometry.- Chapter 3. Redox landscape in flow cytometry.- Section 2. Applications in basic sciences.- Chapter 4. Unravelling biological complexities through flow cytometric applications.- Chapter 5. Illuminating cell dynamics: the versatility of flow cytometry in molecular biology analysis.- Chapter 6. Instrumental role of flow cytometry in molecular biology advancements.- Chapter 7. Flow cytometry: revolutionizing cellular analysis in spectral and microfluidic dimensions.- Chapter 8. Immunological kaleidoscope: flow cytometry's colorful insight into cells.- Chapter 9. Flow cytometric immunophenotyping of leukocytes and neutrophils subsets in bone marrow, peripheral blood and liver tissue.- Chapter 10. Flow cytometric analysis of plant nuclear DNA content in plant homogenates.- Chapter 11. Flow cytometry in microbiological sciences.- Section 3. Applications in clinical and transplantation sciences.- Chapter 12. Applications of flow cytometry in scientific research and the clinic.- Chapter 13. Flow cytometric monitoring of the redox status in health and disease.- Chapter 14. multi-color flow cytometry in medical research: a versatile tool to evaluate the rare immune cells in the pathogenesis of chronic viral infections.- Chapter 15. Flow cytometry approach to differentiate resident versus infiltrated macrophages in atherosclerosis.- Chapter 16. Cytokine analysis in leukemia and minimal residual disease (mrd) detection through flow cytometry.- Chapter 17. Flow cytometry in transplantation.- Chapter 18. Applications of flow cytometry in transplant medicine.- Chapter 19. Flow cytometry based residual disease monitoring in haematolymphoid neoplasm.- Chapter 20. Clinical significance of flow cytometry in solid organ transplantation.- Section 4. Translational, stem cells and regenerative studies.- Chapter 21. An introduction to multiparametric flow cytometry in translational research.- Chapter 22. Stem cels and micro vesicles analysis.- Chapter 23. Translational flow cytometry for repopulating cells in regenerative medicine.- Section 5.- Chapter 24. Flow cytometry in drug design and delivery.- Chapter 25. Flow cytometry in toxicology:illuminating cellular responses.- Section 6. Flow cytometry and environmental studies.- Chapter 26. Exploring the role and potential of flow cytometry in advancing environmental research.- Chapter 27. Role of flow cytometry in the analysis of micro/nano plastics.
