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    Immuno-oncology and immunotherapy Part C

    Immuno-oncology and immunotherapy Part C by Galluzzi, Lorenzo; Bloy, Norma; Charpentier, Maud;

    Series: Methods in Cell Biology; 191;

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    Product details:

    • Publisher Academic Press
    • Date of Publication 16 January 2025

    • ISBN 9780443296208
    • Binding Hardback
    • No. of pages370 pages
    • Size 228x152 mm
    • Weight 720 g
    • Language English
    • 679

    Categories

    Long description:

    Immuno-oncology and immunotherapy, Part C, Volume 191 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Extraction and quantification of histones from human cells, Expression and characterization of Phosphatidylserine-targeting antibodies for biochemical and therapeutic applications, ILC differentiation from HSCs in vitro, Methods to expand human Treg cells and assay their function, Monitoring rapid activation of human gamma/delta T cells by multicolor flow cytometry, Methods to induce T cell exhaustion in vitro, Ex vivo assessment of human neutrophil motility and migration, and much more.

    Additional chapters focus on Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors, Deciphering human blood and tumor neutrophil heterogeneity: Methods for isolation and assessing suppression of T-cell proliferation, Splenocyte anticancer citotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice, Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: a method to confirm immunogenic cell death and assess its therapeutic effectiveness, and much more.

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    Table of Contents:

    1. Simplified acid extraction and quantification of histones in human tumor cells

    Lourdes Hontecillas-Prieto, Daniel J. García-Domínguez, Rocío Flores-Campos; Juan Antonio Flores, Antonio Pérez-Pérez, Luis de la Cruz-Merino, Víctor Sánchez-Margalet and Nabil Hajji

    2. Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications

    Varsha Gadiyar, David Calianese, Rachael Pulica, Christopher Varsanyi, Ziren Wang, Ahmed Aquib, Alok Choudhary and Raymond B. Birge

    3. In vitro ILC differentiation from human HSCs

    Silvia Santopolo, Cecilia Ciancaglini, Francesca Romana Mariotti, Lorenzo Moretta and Linda Quatrini

    4. Optimizing protocols for human regulatory T cell isolation, expansion and functional characterization

    Sánchez-Moreno Inés, Martín-Otal Celia, Lasarte Juan José and Lozano Teresa

    5. (1)Specific selection of stimulation-responsive ?? T cells utilizing a short-term activation assay

    Christian Peters, Jara Simeonov, Daniel Gombert and Dieter Kabelitz

    6. Generation of human and murine exhausted CD8+ T cells in vitro

    Rosa Ana Lacalle, Raquel Blanco, Rebeca García-Lucena and Santos Ma?es

    7. Ex vivo assessment of human neutrophil motility and migration

    Noor A.M. Bakker, Claudia Burrello and Karin E. de Visser

    8. Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors

    Anna-Jasmina Donaubauer, Ilka Scheer, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey

    9. Deciphering neutrophil heterogeneity in human blood and tumors: Methods for isolating neutrophils and assessing their effect on T-cell proliferation

    Nicolas Delhez, Frank Aboubakar Nana, Camille Houbion, Alexandre Bayard, Annika Bruger, Christophe Vanhaver, Sven Brandau, Pierre van der Bruggen and Thibault Hirsch

    10. Splenocytes antitumor cytotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice

    Kenny Misael Calvillo-Rodriguez, Ana Luisa Rivera-Lazarin, Reyes Tamez-Guerra, Ana Carolina Martinez-Torres and Cristina Rodriguez-Padilla

    11. Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: A method to confirm immunogenic cell death and assess its therapeutic effectiveness

    Kenny Misael Calvillo-Rodriguez, Maria Norma Gonzalez-Flores, Reyes Tamez-Guerra,Cristina Rodriguez-Padilla, Marilena Antunes-Ricardo and Ana Carolina Martinez-Torres

    12. Single-cell RNA flow cytometry to assess intratumoral production of cytokines/chemokines

    Khiem C. Lam and Romina S. Goldszmid

    13. Multidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry

    Marieke E. Ijsselsteijn and Noel FCC de Miranda

    14. Evaluation of lymphocyte infiltration into cancer spheroids by immunofluorescent staining and 3D imaging

    Mireia Cruz De los Santos and Andreas Lundqvist

    15. Quantitative pre-clinical imaging of hypoxia and vascularity using MRI and PET Georgia Kanli, Selma Boudissa, Radovan Jirik, Tom Adamsen, Heidi Espedal, Hans-Olav Rolfsnes, Frits Alan Thorsen, Jesus Pacheco Torres, Bassam Janji and Olivier Keunen

    16. Retrovirus-based manufacturing of chimeric antigen receptor-modified T cells for cancer therapy research

    Sophia Stock, Luisa Fertig, Vivien D. Menkhoff, Thaddäus Strzalkowski, Manuel Caruso and Sebastian Kobold

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