
Meiosis
Series: Methods in Molecular Biology; 1471;
- Publisher's listprice EUR 123.04
-
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- Discount 8% (cc. 4 175 Ft off)
- Discounted price 48 018 Ft (45 731 Ft + 5% VAT)
52 193 Ft
Availability
Estimated delivery time: In stock at the publisher, but not at Prospero's office. Delivery time approx. 3-5 weeks.
Not in stock at Prospero.
Why don't you give exact delivery time?
Delivery time is estimated on our previous experiences. We give estimations only, because we order from outside Hungary, and the delivery time mainly depends on how quickly the publisher supplies the book. Faster or slower deliveries both happen, but we do our best to supply as quickly as possible.
Product details:
- Edition number Softcover reprint of the original 2nd ed. 2017
- Publisher Humana
- Date of Publication 21 June 2018
- Number of Volumes 1 pieces, Previously published in hardcover
- ISBN 9781493981694
- Binding Paperback
- No. of pages337 pages
- Size 254x178 mm
- Weight 662 g
- Language English
- Illustrations 14 Illustrations, black & white; 45 Illustrations, color 0
Categories
Short description:
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Long description:
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Table of Contents:
Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe.- Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.- Ribosome Profiling for the Analysis of Translation During Yeast Meiosis.- Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation.- Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora.- High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.- In Vivo Imaging of Budding Yeast Meiosis.- Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I.- Flow Cytometry for theIsolation and Characterization of Rodent Meiocytes.- Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH.- Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy.- Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes.- Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes.- Drosophila Male Meiosis.- Analysis of Chromatin Dynamics During Drosophila Spermatogenesis.- Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model.- A Computational Approach to Study Gene Expression Networks.
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