Hosszú leírás:

Immuno-oncology and immunotherapy, Part C, Volume 191 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Extraction and quantification of histones from human cells, Expression and characterization of Phosphatidylserine-targeting antibodies for biochemical and therapeutic applications, ILC differentiation from HSCs in vitro, Methods to expand human Treg cells and assay their function, Monitoring rapid activation of human gamma/delta T cells by multicolor flow cytometry, Methods to induce T cell exhaustion in vitro, Ex vivo assessment of human neutrophil motility and migration, and much more.

Additional chapters focus on Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors, Deciphering human blood and tumor neutrophil heterogeneity: Methods for isolation and assessing suppression of T-cell proliferation, Splenocyte anticancer citotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice, Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: a method to confirm immunogenic cell death and assess its therapeutic effectiveness, and much more.

Tartalomjegyzék:

1. Simplified acid extraction and quantification of histones in human tumor cells

Lourdes Hontecillas-Prieto, Daniel J. García-Domínguez, Rocío Flores-Campos; Juan Antonio Flores, Antonio Pérez-Pérez, Luis de la Cruz-Merino, Víctor Sánchez-Margalet and Nabil Hajji

2. Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications

Varsha Gadiyar, David Calianese, Rachael Pulica, Christopher Varsanyi, Ziren Wang, Ahmed Aquib, Alok Choudhary and Raymond B. Birge

3. In vitro ILC differentiation from human HSCs

Silvia Santopolo, Cecilia Ciancaglini, Francesca Romana Mariotti, Lorenzo Moretta and Linda Quatrini

4. Optimizing protocols for human regulatory T cell isolation, expansion and functional characterization

Sánchez-Moreno Inés, Martín-Otal Celia, Lasarte Juan José and Lozano Teresa

5. (1)Specific selection of stimulation-responsive ?? T cells utilizing a short-term activation assay

Christian Peters, Jara Simeonov, Daniel Gombert and Dieter Kabelitz

6. Generation of human and murine exhausted CD8+ T cells in vitro

Rosa Ana Lacalle, Raquel Blanco, Rebeca García-Lucena and Santos Ma?es

7. Ex vivo assessment of human neutrophil motility and migration

Noor A.M. Bakker, Claudia Burrello and Karin E. de Visser

8. Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors

Anna-Jasmina Donaubauer, Ilka Scheer, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey

9. Deciphering neutrophil heterogeneity in human blood and tumors: Methods for isolating neutrophils and assessing their effect on T-cell proliferation

Nicolas Delhez, Frank Aboubakar Nana, Camille Houbion, Alexandre Bayard, Annika Bruger, Christophe Vanhaver, Sven Brandau, Pierre van der Bruggen and Thibault Hirsch

10. Splenocytes antitumor cytotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice

Kenny Misael Calvillo-Rodriguez, Ana Luisa Rivera-Lazarin, Reyes Tamez-Guerra, Ana Carolina Martinez-Torres and Cristina Rodriguez-Padilla

11. Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: A method to confirm immunogenic cell death and assess its therapeutic effectiveness

Kenny Misael Calvillo-Rodriguez, Maria Norma Gonzalez-Flores, Reyes Tamez-Guerra,Cristina Rodriguez-Padilla, Marilena Antunes-Ricardo and Ana Carolina Martinez-Torres

12. Single-cell RNA flow cytometry to assess intratumoral production of cytokines/chemokines

Khiem C. Lam and Romina S. Goldszmid

13. Multidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry

Marieke E. Ijsselsteijn and Noel FCC de Miranda

14. Evaluation of lymphocyte infiltration into cancer spheroids by immunofluorescent staining and 3D imaging

Mireia Cruz De los Santos and Andreas Lundqvist

15. Quantitative pre-clinical imaging of hypoxia and vascularity using MRI and PET Georgia Kanli, Selma Boudissa, Radovan Jirik, Tom Adamsen, Heidi Espedal, Hans-Olav Rolfsnes, Frits Alan Thorsen, Jesus Pacheco Torres, Bassam Janji and Olivier Keunen

16. Retrovirus-based manufacturing of chimeric antigen receptor-modified T cells for cancer therapy research

Sophia Stock, Luisa Fertig, Vivien D. Menkhoff, Thaddäus Strzalkowski, Manuel Caruso and Sebastian Kobold